8-hydroxy-2’-deoxyguanosine (8-OHdG) is considered a pivotal biomarker of oxidative DNA damage associated with mutagenesis and carcinogenesis. Common methods for detecting 8-OHdG in DNA samples can be time consuming, costly and lack the ability to use intact DNA from cells or tissues. Thankfully, our ELISA-like EpiQuik 8-OHdG DNA Damage Quantification Direct Kit can detect and quantify oxidative DNA damage (8-OHdG) directly from DNA in less than 4 hours.
- Extremely fast, less than 4-hour procedure• Detection limit as low as 2 pg of 8-OHdG
- No cross-reactivity to 8-OHdG analogues
Detect In Situ DNA Damage
Phosphorylation of histone H2AX to form rH2AX is important for the coordination of signaling and repair activities. Use our EpiQuik In Situ DNA Damage Assay Kit to detect DNA damage or apoptosis by measuring for H2AX phosphorylation at serine 139 in situ.
- Fast 3-hour procedure• Measure in situ histone H2AX phosphorylation without preparing cell lysates
- Flexible microplate format for high throughput analysis of agents that increases or inhibits DNA damage
- Simple, reliable, and consistent assay conditions
Featured Open Access Article
Researchers examined the effects of cigarette smoke condensate (CSC) on monocyte-derived exosomes and the subsequent influence on HIV-1 replication. They found that exosomes secreted from CSC-exposed monocytes, especially from uninfected cells, were altered in a way that allowed them to exhibit a protective effect against toxicity and viral replication. Their results indicate that exosomes may play a novel role in tobacco-mediated HIV-1 pathogenesis. In the study, 8-OHdG DNA damage was measured using our EpiQuik 8-OHdG DNA Damage Quantification Direct Kit.